Fractionation of sialylated oligosaccharides, glycopeptides, and glycoproteins on immobilized elderberry (Sambucus nigra L.) bark lectin

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Fractionation of sialylated oligosaccharides, glycopeptides, and glycoproteins on immobilized elderberry (Sambucus nigra L.) bark lectin.

A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of standard compounds. Oligosaccharides, glycopeptides, or glycoproteins containi...

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Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark.

A second lectin (SNA-II) has been isolated from elderberry (Sambucus nigra L.) bark by affinity chromatography on immobilized asialo-glycophorin. This lectin is a blood group nonspecific glycoprotein containing 7.8% carbohydrate and which is rich in asparagine/aspartic acid, glutamine/glutamic acid, glycine, valine, and leucine. Gel filtration on Superose 12 gave a single symmetrical peak corre...

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Sambucus nigra (Elderberry) Monograph

Introduction Sambucus nigra, or European elder, is a tall tree-like shrub, native to Europe, Asia, and North Africa, and naturalized in the United States. Various parts of the elder have long been used in traditional medicine as a diaphoretic, diuretic, astringent, laxative, and emetic. The berries were used traditionally as a food to make elderberry wine and pies, and as a flavoring or dye. Cu...

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Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin.

The carbohydrate-binding specificity of Aleuria aurantia lectin was investigated by analyzing the behavior of a variety of fucose-containing oligosaccharides on an A. aurantia lectin-Sepharose column. Studies with complex-type oligosaccharides obtained from various glycoproteins by hydrazinolysis and their partial degradation fragments indicated that the presence of the alpha-fucosyl residue li...

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Polyphenolic content, antiradical activity, stability and microbiological quality of elderberry (Sambucus nigra L.) extracts.

BACKGROUND The pharmaceutical and food industries expect detailed knowledge on the physicochemical properties of elderberry fruit extracts, their stability and microbiological quality, as well as the polyphenol content in elderberry cultivars. The characteristics of the extracts might be additionally modified by citric acid, which improves the stability of anthocyanins and protects processed fr...

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ژورنال

عنوان ژورنال: Archives of Biochemistry and Biophysics

سال: 1987

ISSN: 0003-9861

DOI: 10.1016/0003-9861(87)90074-9